Photoacoustic lymphatic imaging with high spatial-temporal resolution

Photoacoustic lymphatic imaging with high spatial-temporal resolution.

Despite its critical function in coordinating the egress of inflammatory and immune cells out of tissues and maintaining fluid balance, the causative role of lymphatic network dysfunction in pathological settings is still understudied. Engineered-animal models and better noninvasive high spatial-temporal resolution imaging techniques in both preclinical and clinical studies will help to improve...

High-resolution photoacoustic imaging with focused laser and ultrasonic beams.

We report a photoacoustic imager that utilizes a focused laser beam in combination with a 20 MHz ultrasound focusing transducer to obtain micron-resolution tissue images over a long working distance. The imager is based on a ring transducer that combines ultrasonic and laser beams collinearly and confocally in a monolithic element. The combination of focused laser beam and short pulse irradiati...

High temporal resolution imaging of spatial working memory

A substantial literature examining the neural correlates of workingmemory using functional magnetic resonance imaging (fMRI) has revealed the involvement of a predominantly fronto–parietal network in the maintenance and manipulation of information in working memory. However, the temporal dynamics of activity in this system cannot be revealed by fMRI at the sub-second level. We employed magnetoe...

High Spatial Resolution Lipid Imaging

High-resolution photoacoustic microscope for rat brain imaging in vivo

A reflection-mode photoacoustic microscope (PAM) for rat brain imaging in vivo is constructed. A pulsed laser is used as an excitation source, and a focused ultrasound transducer is adopted to collect the photoacoustic signal. Raster scanning is applied to acquire three-dimensional (3D) data. The obtained measurements of the lateral and axial resolutions of the microscope are 45 and 15 μm, resp...